博来霉素对多巴胺-β-羟化酶的光标记

Photolabelling of dopamine-beta-hydroxylase by bleomycin.

作者信息

Ratwatte H A, Douglas K T

出版信息

Biochem Biophys Res Commun. 1983 Apr 15;112(1):273-8. doi: 10.1016/0006-291x(83)91826-0.

Abstract

Irradiation of a mixture of dopamine-beta-hydroxylase and bleomycin with light of wavelengths greater than 300 nm. leads to irreversible inhibition which is not prevented in the presence of tyramine. Prephotolysis of bleomycin followed by incubation (in the dark) with dopamine-beta-hydroxylase did not lead to irreversible inhibition. Thus bleomycin is a photolabel of this enzyme.

摘要

用波长大于300纳米的光照射多巴胺-β-羟化酶和博来霉素的混合物，会导致不可逆抑制，在酪胺存在的情况下这种抑制无法被阻止。博来霉素预光解后，再与多巴胺-β-羟化酶在黑暗中孵育，不会导致不可逆抑制。因此，博来霉素是这种酶的光标记物。