Kasturi K, Fleming J, Harrison P
Exp Cell Res. 1983 Mar;144(1):241-7. doi: 10.1016/0014-4827(83)90462-7.
A panel of nine monoclonal antibodies against the characteristic erythrocyte membrane protein spectrin has been isolated. One antibody reacts with both the 240 000 and 220 000 D alpha- and beta-subunits of spectrin after denaturation. The same antibody reacts with a 240 000 D protein present in various hemopoietic and other cell lines, as well as some smaller polypeptides, as established by western blotting and immunoautoradiography. These results indicate that the alpha- and beta-subunits of spectrin, a polypeptide of 240 000, and some smaller polypeptides present in non-erythroid cell types possess a considerable region of sequence homology, but it is not yet clear just how extensively the spectrin-like molecules and other polypeptides are related.
已分离出一组针对特征性红细胞膜蛋白血影蛋白的九种单克隆抗体。一种抗体在变性后能与血影蛋白的240000和220000Dα-和β-亚基发生反应。通过蛋白质印迹法和免疫放射自显影证实，同一抗体能与存在于各种造血细胞系和其他细胞系中的一种240000D蛋白以及一些较小的多肽发生反应。这些结果表明，血影蛋白的α-和β-亚基、一种240000的多肽以及非红细胞类型中存在的一些较小的多肽具有相当大的序列同源区域，但血影蛋白样分子与其他多肽之间的相关程度究竟如何尚不清楚。
