McLennan I S
Dev Biol. 1983 May;97(1):222-8. doi: 10.1016/0012-1606(83)90079-9.
The differentiation of myotubes into fiber types was studied by examining the ATPase staining characteristics of chicken embryo thigh muscles. Two distinct fiber types, designated type IEMB and IIEMB, could be distinguished as early as stage 29. Paralysis of the embryo with d-tubocurarine prevented the differentiation of type IEMB but not type IIEMB characteristics. The two embryonic fiber types differed from each other, and mature type I and II fibers, in the acid and alkali labilities of their ATPases. Myotubes which were type IEMB at stage 29 matured into type I fibers, whereas those which were type IIEMB predominantly but not exclusively developed into type II fibers. The process of maturation involved sequential changes in the staining characteristics of the myotubes. Thus, the ultimate fiber type of a myotube can be detected long before it expresses its mature characteristics.
通过检查鸡胚大腿肌肉的ATP酶染色特性，研究了肌管向纤维类型的分化。早在第29阶段就可以区分出两种不同的纤维类型，分别命名为IEMB型和IIEMB型。用d -筒箭毒碱使胚胎麻痹可阻止IEMB型的分化，但不影响IIEMB型的特征。这两种胚胎纤维类型在其ATP酶的酸碱性稳定性方面彼此不同，也与成熟的I型和II型纤维不同。在第29阶段为IEMB型的肌管成熟为I型纤维，而那些主要但并非唯一为IIEMB型的肌管则发育为II型纤维。成熟过程涉及肌管染色特性的一系列变化。因此，在肌管表达其成熟特征之前很久就可以检测到其最终的纤维类型。
