Shelton G D, Bandman E
J Muscle Res Cell Motil. 1985 Aug;6(4):435-46. doi: 10.1007/BF00712581.
The myosin isozyme composition of the lateral gastrocnemius muscle of the chicken leg was investigated during various stages of development utilizing non-denaturing pyrophosphate gel electrophoresis, two-dimensional gel electrophoresis and peptide mapping techniques. An unusual isoform pattern for fast myosin in the lateral gastrocnemius muscle of the adult chicken leg was demonstrated which consisted of a predominance of myosin homodimers and lesser amounts of myosin heterodimer. In addition, a different myosin heavy chain isoform was present in the adult chicken lateral gastrocnemius muscle when compared to other adult fast-twitch muscles. While the adult lateral gastrocnemius muscle contained a different myosin heavy chain isoform from other adult fast-twitch muscles, the embryonic lateral gastrocnemius muscle contained a myosin heavy chain identical to that of the embryonic pectoralis major.
利用非变性焦磷酸凝胶电泳、二维凝胶电泳和肽图分析技术，对鸡腿外侧腓肠肌在不同发育阶段的肌球蛋白同工酶组成进行了研究。结果表明，成年鸡腿外侧腓肠肌中快速肌球蛋白的同工型模式不同寻常，主要由肌球蛋白同型二聚体和少量肌球蛋白异型二聚体组成。此外，与其他成年快肌相比，成年鸡外侧腓肠肌中存在不同的肌球蛋白重链同工型。虽然成年外侧腓肠肌含有与其他成年快肌不同的肌球蛋白重链同工型，但胚胎外侧腓肠肌所含的肌球蛋白重链与胚胎期胸大肌的相同。
