Bonnet K A, Bondy S C
Exp Brain Res. 1976 Sep 24;26(2):185-91. doi: 10.1007/BF00238282.
Autoradiograms of the optic tectum were made 8 days after monocular injection of 3H-uridine in 3 day-old chicks. Electron microscopic analysis of grain density over morphological structures in various layers of the optic tectum contralateral to the injected eye, was carried out. This revealed that the highest concentration of labeled RNA was within the axons of the more superficial layers of the tectum. A lesser density of grains was observed over glial cells, neuronal perikarya and dendrites. In addition, the bulk of the total grains counted were over axons. In lower tectal layers, radioactivity was predominantly over unmyelinated axons and glial elements. High molecular weight RNA appears to be transported within the axons of the optic nerve toward their terminations which are largely in the outer layer of the optic tract.
在3日龄雏鸡单眼注射3H-尿苷8天后，制作了视顶盖的放射自显影片。对注射眼对侧视顶盖各层形态结构上的颗粒密度进行了电子显微镜分析。结果显示，标记RNA的最高浓度位于顶盖较浅层的轴突内。在神经胶质细胞、神经元胞体和树突上观察到的颗粒密度较低。此外，计数的总颗粒大部分位于轴突上。在顶盖较深层，放射性主要位于无髓轴突和神经胶质成分上。高分子量RNA似乎在视神经轴突内朝着其主要位于视束外层的终末运输。
