Romhányi G
Histochemistry. 1983;77(1):133-9. doi: 10.1007/BF00496644.
The ultrastructural organization of the elastic fibres of bovine ligamentum nuchae is still controversial (Kádár 1979; Sandberg 1976; Sandberg et al. 1981; Quintarelli et al. 1973; Gosline 1976). Polarization microscopy demonstrated convincingly, contrary to a recent negative report (Aaron and Gosline 1980), an ordered birefringent micellar texture (Schmidt 1939; Romhányi 1965; Fischer 1979). The three-banded anisotropic pattern of the fibres can be explained by the optical interference of two micellar textures of opposite orientation, one arranged circularly in the surface layer and the other oriented longitudinally in the axial core of the fibres (Romhányi 1965). Analysis of the underlying molecular mechanism for the anisotropic staining reaction of these fibres with Congo red led to the conclusion that this is due to a definite structural order of specific cross-linking segments within the random network of elastin. The anisotropic staining reaction of the elastic fibres with Congo red is described as the most simple method to demonstrate the three-banded birefringent pattern of ligamentum nuchae elastic fibres even in histologic sections.
牛项韧带弹性纤维的超微结构组织仍存在争议（卡达尔，1979年；桑德伯格，1976年；桑德伯格等人，1981年；昆塔雷利等人，1973年；戈斯林，1976年）。与最近的一份否定报告（亚伦和戈斯林，1980年）相反，偏光显微镜令人信服地证明了一种有序的双折射胶束纹理（施密特，1939年；罗曼伊，1965年；菲舍尔，1979年）。纤维的三带各向异性图案可以通过两种相反取向的胶束纹理的光学干涉来解释，一种在表层呈圆形排列，另一种在纤维的轴向核心纵向取向（罗曼伊，1965年）。对这些纤维与刚果红的各向异性染色反应的潜在分子机制进行分析后得出结论，这是由于弹性蛋白随机网络内特定交联段的确定结构顺序所致。弹性纤维与刚果红的各向异性染色反应被描述为即使在组织学切片中也能显示项韧带弹性纤维三带双折射图案的最简单方法。
