Costantino-Ceccarini E, Suzuki K
J Biol Chem. 1978 Jan 25;253(2):340-2.
A heat-stable factor has been extracted from the microsomal membranes of rat brain that inhibits the activities of rat brain ceramide galactosyl- and glucosyltransferases. It is nondialyzable, susceptible to proteolytic enzymes but resistant to DNase and RNase, and has no effect on lysosomal hydrolytic enzymes. The factor could also be prepared from microsomes of bovine, mouse, and rabbit brains and in much lower concentrations, from systemic organs of rats. This inhibitor might play a role in the regulation of ceramide glycosyltransferases in vivo.
已从大鼠脑微粒体膜中提取出一种热稳定因子，该因子可抑制大鼠脑内神经酰胺半乳糖基转移酶和葡萄糖基转移酶的活性。它不能透析，易受蛋白水解酶作用，但对脱氧核糖核酸酶和核糖核酸酶有抗性，且对溶酶体水解酶无影响。该因子也可从牛、小鼠和兔脑的微粒体中制备，并且能以低得多的浓度从大鼠的全身器官中制备。这种抑制剂可能在体内神经酰胺糖基转移酶的调节中发挥作用。
