Ohtsuki K
Tex Rep Biol Med. 1981;41:493-8.
The experimental data reviewed in this paper suggest that the inhibition of viral polypeptide chain initiation in IFN-treated cells involves the phosphorylation of eIF-2 alpha and intermediate factors (65K and 67K ribosomal proteins) by an IFN-induced dsRNA-dependent ribosomal kinase. However, the discrimination mechanism between viral and host cell mRNAs at the translational level remain to be elucidated, because the mechanism is very complex. IFN will induce, for example, preferential digestion of viral mRNAs by 2',5'-oligo A activated endoribonuclease, will impair tRNAs and elongation factors for the protein synthesis, and will decrease methylation of viral mRNAs, and they are involved in the discrimination mechanism at translational level. The activation of membrane-associated kinase activity by IFNs is very interesting because its among the earliest recognized biochemical events induced in cells and may play an important role in IFN-induced host or cell defense. Further studies focusing on the biochemical roles of the membrane-associated kinase in IFN-treated cells may provide evidence for understanding the biochemical mechanism of cell activation by IFNs.
本文中所回顾的实验数据表明，在经干扰素处理的细胞中，病毒多肽链起始的抑制涉及到真核起始因子2α（eIF-2α）以及中间因子（65K和67K核糖体蛋白）被一种干扰素诱导的双链RNA依赖性核糖体激酶磷酸化。然而，在翻译水平上病毒和宿主细胞mRNA之间的识别机制仍有待阐明，因为该机制非常复杂。例如，干扰素会诱导2',5'-寡聚腺苷酸激活的核糖核酸内切酶对病毒mRNA进行优先消化，会损害蛋白质合成所需的转运RNA（tRNA）和延伸因子，还会降低病毒mRNA的甲基化，而这些都参与了翻译水平上的识别机制。干扰素对膜相关激酶活性的激活非常有趣，因为这是在细胞中最早被认识到的生化事件之一，并且可能在干扰素诱导的宿主或细胞防御中发挥重要作用。进一步聚焦于膜相关激酶在经干扰素处理细胞中的生化作用的研究，可能会为理解干扰素激活细胞的生化机制提供证据。
