Kozek W J, Eberhard M L, Raccurt C
Tropenmed Parasitol. 1983 Mar;34(1):33-7.
Comparative morphological studies were conducted at the light microscope level to determine whether any difference could be detected in thin smears, thick smears, or Knott samples, between Colombian (simuliid-transmitted) and Haitian (culicoid-transmitted) Mansonella ozzardi microfilaria. Results indicated that the microfilariae from these two geographical areas are essentially identical. The small differences observed in the dimensions of microfilariae in thick and in thin smears can be attributed to different preparative procedures. The consistent morphological differences, noted in PAS-hematoxylin-stained microfilariae, from Knott smears, was that the central body was thinner and stained less intensely with the Schiff reagent in the Haitian than in the Colombian microfilariae. Although these observations suggest a physiological difference between these two forms, additional studies are needed to exclude the possibility that these differences are not indications of the age or a state of physiological activity of the microfilariae.
进行了光学显微镜水平的比较形态学研究，以确定在哥伦比亚（蚋传播）和海地（蠓传播）的奥氏曼森线虫微丝蚴的薄涂片、厚涂片或诺特样本之间是否能检测到任何差异。结果表明，来自这两个地理区域的微丝蚴基本相同。在厚涂片和薄涂片中观察到的微丝蚴尺寸的微小差异可归因于不同的制备程序。在诺特涂片的过碘酸-苏木精染色微丝蚴中观察到的一致形态学差异是，海地微丝蚴的中央体比哥伦比亚微丝蚴的更细，用席夫试剂染色的强度更低。尽管这些观察结果表明这两种形态之间存在生理差异，但还需要进一步研究以排除这些差异不是微丝蚴年龄或生理活动状态指标的可能性。
