在有或没有甲状旁腺激素存在的情况下，前列腺素对培养软骨细胞中3',5'-单磷酸腺苷积累的刺激作用。

Prostaglandin stimulation of adenosine 3',5'-monophosphate accumulation in cultured chondrocytes in the presence or absence of parathyroid hormone.

作者信息

Kinoshita M, Kato Y, Tsuji M, Kono T, Hiraki Y, Suzuki F

出版信息

Biochim Biophys Acta. 1983 Jun 9;757(3):324-31. doi: 10.1016/0304-4165(83)90058-2.

DOI:10.1016/0304-4165(83)90058-2

Abstract

The effect of prostaglandin analogues on the cyclic AMP level in cultured chondrocytes were examined. Prostaglandin E1 at 0.4 to 30 microM, increased the intracellular concentration of cyclic AMP in chondrocytes. Its effect was rapid, being evident within 1 min and reaching a maximum in 10 to 20 min. The maximum level was sustained until 30 min after its addition and then decreased gradually. Prostaglandin D2 and E2 also increased the cyclic AMP level in chondrocytes, but they had less effect than prostaglandin E1. Prostaglandin A1 had no effect on the nucleotide level in chondrocytes, although they markedly increased the level in fibroblasts. The time course of stimulation of cyclic AMP accumulation in chondrocytes by prostaglandin E1, D2 or E2 was quite different from that by parathyroid hormone (PTH): the effect of prostaglandin was slower and more sustained than that of PTH. PTH potentiated the effect of prostaglandin E1, E2, or D2 on the cyclic AMP level in chondrocytes and that the combined effects of prostaglandin and PTH were more than additive. Addition of an inhibitor of cyclic nucleotide phosphodiesterase with prostaglandin, PTH or both produced a synergistic effect on the accumulation of cyclic AMP in the chondrocytes. These findings suggest that prostaglandin E1, E2, and D2 increase the synthesis of cyclic AMP and that the combined effect of the prostaglandins and PTH on the cyclic AMP level in chondrocytes is partly attributed to the synergistic synthesis of cyclic AMP in the cells.

摘要

研究了前列腺素类似物对培养软骨细胞中环磷酸腺苷（cAMP）水平的影响。0.4至30微摩尔的前列腺素E1可增加软骨细胞内cAMP的浓度。其作用迅速，在1分钟内即可显现，10至20分钟达到最大值。最大水平持续至添加后30分钟，然后逐渐下降。前列腺素D2和E2也可增加软骨细胞中的cAMP水平，但作用比前列腺素E1小。前列腺素A1对软骨细胞中的核苷酸水平无影响，尽管它们可显著提高成纤维细胞中的水平。前列腺素E1、D2或E2刺激软骨细胞中cAMP积累的时间进程与甲状旁腺激素（PTH）的不同：前列腺素的作用比PTH慢且更持久。PTH增强了前列腺素E1、E2或D2对软骨细胞中cAMP水平的作用，且前列腺素和PTH的联合作用大于相加作用。与前列腺素、PTH或两者一起添加环核苷酸磷酸二酯酶抑制剂对软骨细胞中cAMP的积累产生协同作用。这些发现表明，前列腺素E1、E2和D2可增加cAMP的合成，且前列腺素和PTH对软骨细胞中cAMP水平的联合作用部分归因于细胞中cAMP的协同合成。