Brown A, Feizi T, Gooi H C, Embleton M J, Picard J K, Baldwin R W
Biosci Rep. 1983 Feb;3(2):163-70. doi: 10.1007/BF01121947.
A monoclonal antibody C14/1/46/10 showing preferential binding to membranes of human colorectal carcinomas over normal colon mucosae was obtained by immunization of mice with extra-nuclear membranes of a human colonic adenoma. Binding and inhibition of binding assays using blood cells or glycoproteins with known blood-group activities indicated that the antibody recognizes a carbohydrate antigen co-existing with the blood-group-H determinant: Fuc alpha 1 leads to 2 Gal. Inhibition assays with structurally defined oligosaccharides showed that the antigenic determinant involves difucosylated Type-2-blood-group chains with the structure: (formula; see text)
通过用人结肠腺瘤的核外膜免疫小鼠，获得了一种单克隆抗体C14/1/46/10，该抗体与人结肠直肠癌膜的结合优于正常结肠黏膜。使用具有已知血型活性的血细胞或糖蛋白进行的结合和结合抑制试验表明，该抗体识别与血型H决定簇共存的碳水化合物抗原：岩藻糖α1连接到2半乳糖。用结构明确的寡糖进行的抑制试验表明，抗原决定簇涉及具有以下结构的二岩藻糖基化2型血型链：（分子式；见正文）
