牛肉心亚线粒体颗粒中寡霉素敏感性赋予蛋白结合位点的滴定
Titration of the binding sites for the oligomycin-sensitivity conferring protein in beef heart submitochondrial particles.
作者信息
Dupuis A, Satre M, Vignais P V
出版信息
FEBS Lett. 1983 May 30;156(1):99-102. doi: 10.1016/0014-5793(83)80256-7.
PMID:6189744
Abstract
The binding parameters of the oligomycin-sensitivity conferring protein (OSCP) in inside-out particles from beef heart mitochondria have been tested by means of two assays, the oligomycin-sensitive ATP-Pi exchange, and the oligomycin-sensitive ATP hydrolysis. The total number of OSCP binding sites in A particles was equal to 220 pmol/mg particle protein. Each mole of ATPase active site was able to bind 1.1 +/- 0.5 mol OSCP with Kd 1.7 nM.
摘要
通过两种测定方法，即对寡霉素敏感的ATP-无机磷酸交换和对寡霉素敏感的ATP水解，对牛肉心线粒体的内翻颗粒中寡霉素敏感性赋予蛋白（OSCP）的结合参数进行了测试。A颗粒中OSCP结合位点的总数等于220 pmol/mg颗粒蛋白。每摩尔ATP酶活性位点能够结合1.1±0.5摩尔OSCP，解离常数为1.7 nM。
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