Wells M A, Daniel S, Djeu J Y, Kiley S C, Ennis F A
J Immunol. 1983 Jun;130(6):2908-14.
Immune spleen cells enhanced for influenza-specific cytotoxic activity after exposure to virus-infected stimulator cells in vitro effect recovery when transferred to nude and immunocompetent mice with influenza pneumonia (5). This protective effect correlated with the virus-specific cytotoxic activity of the transferred lymphocytes and is removed by treatment with anti-0 serum and complement. The experiments presented here indicate that spleen cells taken directly from mice undergoing a primary or secondary infection are less protective than immune spleen cells that are restimulated in vitro before transfer. This decreased ability to clear pulmonary virus and effect survival correlated with their relatively lower levels of influenza-specific cytotoxicity. Protection did not correlate with the level of natural killer cell activity of transferred cells. The results also indicate the immune spleen cells that are protective are influenza A subtype cross-reactive and are H-2-restricted; H-2d immune spleen cells effected recovery of H-2d but not H-2k challenged mice.
体外暴露于病毒感染的刺激细胞后，免疫脾细胞对流感特异性细胞毒性活性增强，当将其转移至患有流感肺炎的裸鼠和免疫活性小鼠时可实现恢复（5）。这种保护作用与转移淋巴细胞的病毒特异性细胞毒性活性相关，并可通过抗θ血清和补体处理消除。此处呈现的实验表明，直接取自经历初次或二次感染小鼠的脾细胞，其保护作用不如转移前在体外再次刺激的免疫脾细胞。清除肺部病毒和影响存活的能力下降与其相对较低的流感特异性细胞毒性水平相关。保护作用与转移细胞的自然杀伤细胞活性水平无关。结果还表明，具有保护作用的免疫脾细胞是甲型流感亚型交叉反应性的且受H-2限制；H-2d免疫脾细胞可使H-2d但非H-2k攻击的小鼠恢复。
