Saikh K U, Martin J D, Nishikawa A H, Dillon S B
Department of Protein Biochemistry, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406-0939, USA.
Virology. 1995 Dec 20;214(2):445-52. doi: 10.1006/viro.1995.0055.
BALB/c mice immunized with a vaccinia virus recombinant expressing the influenza A virus (A/Udorn/72; subtype H3) hemagglutinin HA2 (H3HA2) induced a strong CD8+ cytotoxic T lymphocyte (CTL) response which was H-2d-restricted. Two peptides, derived from the primary sequence of the H3HA2 and consistent with the predictive motif for Kd-restricted epitopes, were tested for their ability to be presented to the CTLs by P815 cells. Peptides corresponding to the amino acids 93SYNAELLVAL102 and 181GYKDWILWI189 of the HA2 primary sequence sensitized target cells for lysis by the HA2-specific CTLs. Secondary in vitro stimulation with dendritic cells as a source of antigen presenting cells treated with peptide 93-102, or infected with recombinant vaccinia virus (HA2-VACC), induced type A cross-reactive CTLs from A/Udorn/72 immune spleen cells. This CD8+ CTL epitope overlaps with an H3HA2 I-Ad-restricted T helper epitope 96-104 recently reported by others. The H3HA2 epitope described here is the first CTL epitope in the influenza hemagglutinin which is found in all three subtypes of influenza A virus.
用表达甲型流感病毒（A/Udorn/72；H3亚型）血凝素HA2（H3HA2）的痘苗病毒重组体免疫的BALB/c小鼠诱导了强烈的H-2d限制性CD8+细胞毒性T淋巴细胞（CTL）应答。从H3HA2的一级序列衍生出的、与Kd限制性表位的预测基序一致的两种肽，被检测其被P815细胞呈递给CTL的能力。对应于HA2一级序列氨基酸93SYNAELLVAL102和181GYKDWILWI189的肽使靶细胞对HA2特异性CTL的裂解敏感。用肽93-102处理的树突状细胞作为抗原呈递细胞来源进行体外二次刺激，或用重组痘苗病毒（HA2-VACC）感染，从A/Udorn/72免疫脾细胞诱导出A型交叉反应性CTL。这种CD8+CTL表位与其他人最近报道的H3HA2 I-Ad限制性T辅助表位96-104重叠。这里描述的H3HA2表位是甲型流感病毒所有三种亚型中在流感血凝素中发现的首个CTL表位。
