Measurement of thymosin alpha 1 by disassociation microELISA.
作者信息
Mutchnick M G, Keren D F, Weller F E, McClure J E, Goldstein A L
出版信息
J Immunol Methods. 1983 May 27;60(1-2):53-60. doi: 10.1016/0022-1759(83)90334-4.
PMID:6189912
Abstract
A disassociation microELISA was devised for the estimation of thymosin alpha 1, a chemically characterized thymic polypeptide isolated from bovine thymosin fraction 5. Antiserum to synthetic thymosin alpha 1 was raised in rabbits. Thymosin alpha 1 in liquid phase competed with a solid-phase-bound thymosin alpha 1 for this highly specific antibody. The method is specific, sensitive, reproducible and capable of detecting as little as 100 pg/ml of thymosin alpha 1.
摘要
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