Hafkenscheid J C, Hessels M, van der Hoek E W
J Clin Chem Clin Biochem. 1983 Mar;21(3):167-74. doi: 10.1515/cclm.1983.21.3.167.
The three most important enzymes present in duodenal fluid, alpha-amylase, trypsin and lipase, were determined by two different methods. Various dilution fluids for these determinations were compared. Some kinetic data are described together with the reproducibility of the assays. For alpha-amylase the Phadebas method was compared with an UV method. Trypsin was determined using an immunoreactive trypsin test and a titrimetric method, while lipase was determined with a titrimetric method and a Cu-soap method. There was very good agreement between the two alpha-amylase methods and between the two trypsin determinations. There was also good agreement between both lipase methods. The advantages and disadvantages of all the methods are discussed.
通过两种不同方法测定了十二指肠液中存在的三种最重要的酶，即α-淀粉酶、胰蛋白酶和脂肪酶。比较了用于这些测定的各种稀释液。描述了一些动力学数据以及测定的重现性。对于α-淀粉酶，将Phadebas法与紫外法进行了比较。使用免疫反应性胰蛋白酶试验和滴定法测定胰蛋白酶，而用滴定法和铜皂法测定脂肪酶。两种α-淀粉酶方法之间以及两种胰蛋白酶测定结果之间具有很好的一致性。两种脂肪酶方法之间也具有良好的一致性。讨论了所有方法的优缺点。
