Zweig S E, Shevach E M
Methods Enzymol. 1983;92:66-85. doi: 10.1016/0076-6879(83)92010-4.
Although the use of monoclonal antibodies to characterize the Ia antigen system is still in its infancy, the usefulness of this approach is clearly apparent. Since xenogeneic immunization can be used to generate these antibodies, the chances are good that a greater variety of Ia types and determinants will be detected compared to those detected with conventional alloantisera. In addition, the advantages of unlimited quantity and constant specificity allow a much greater degree of standardization than was previously possible. Finally, monoclonal antibodies open up lines of investigation, such as the examination of correlations between the monoclonal antibody's inhibition of T-cell proliferation and the antibody's corresponding Ia epitope-binding characteristics, that it would be impossible to approach using conventional alloantisera.
尽管使用单克隆抗体来鉴定Ia抗原系统仍处于起步阶段，但这种方法的实用性已明显显现。由于可以利用异种免疫来产生这些抗体，与使用传统同种异体抗血清检测到的Ia类型和决定簇相比，检测到更多种类Ia类型和决定簇的可能性很大。此外，单克隆抗体数量无限且特异性恒定的优点使得标准化程度比以前有了更大提高。最后，单克隆抗体开辟了新的研究途径，例如研究单克隆抗体对T细胞增殖的抑制作用与其相应Ia表位结合特性之间的相关性，而这是使用传统同种异体抗血清无法实现的。
