Burger R, Scher I, Sharrow S O, Shevach E M
Immunology. 1984 Jan;51(1):93-102.
Conventional alloantisera and monoclonal antibodies to guinea-pig Ia antigens were used for analysis of Ia expression by guinea-pig T cells and thymocytes. Indirect immunofluorescent staining was performed with alloantisera or with ascitic fluid as a source of monoclonal antibody followed by flow microfluorometry analysis on the fluorescence activated cell sorter. About 80% of normal, non-activated peritoneal exudate T cells, lymph node T cells and thymocytes expressed Ia antigens. These data are therefore in contrast to studies with human or murine T cells where Ia antigens were shown to be expressed predominantly on activated but not on non-activated T cells. All the reactivity of the anti-Ia alloantisera for strain 2 T cells could be removed by absorption with an Ia-bearing B cell leukaemia, EN-L2C, but not by its Ia-negative variant, BZ-L2C. Thus, the Ia determinants identified on T and B cells are probably identical. One monoclonal antibody, 25E3, which had previously been shown by serologic analysis to react exclusively with an alloantigenic determinant of strain 2 Ia antigens displayed an unusual pattern of reactivity in that it clearly stained strain 13 thymocytes, but not mature strain 13 T or B lymphocytes. The significance of this possible expression of inappropriate Ia determinants by thymocytes remains unclear. This phenomenon might be associated with differentiation processes in the thymus.
使用针对豚鼠Ia抗原的传统同种抗血清和单克隆抗体来分析豚鼠T细胞和胸腺细胞的Ia表达情况。采用同种抗血清或腹水作为单克隆抗体来源进行间接免疫荧光染色，随后在荧光激活细胞分选仪上进行流式微荧光分析。大约80%的正常、未激活的腹腔渗出液T细胞、淋巴结T细胞和胸腺细胞表达Ia抗原。因此，这些数据与对人或鼠T细胞的研究结果形成对比，在人或鼠T细胞研究中，Ia抗原主要在激活的T细胞而非未激活的T细胞上表达。抗Ia同种抗血清对2系T细胞的所有反应性都可通过与携带Ia的B细胞白血病EN-L2C吸收而去除，但不能被其Ia阴性变体BZ-L2C去除。因此，在T细胞和B细胞上鉴定出的Ia决定簇可能是相同的。一种单克隆抗体25E3，先前通过血清学分析显示仅与2系Ia抗原的一种同种抗原决定簇反应，其显示出一种不寻常的反应模式，即它能清晰地染色13系胸腺细胞，但不能染色成熟的13系T或B淋巴细胞。胸腺细胞这种可能表达不适当Ia决定簇的现象的意义仍不清楚。这种现象可能与胸腺中的分化过程有关。
