Milner E C, Capra J D
Mol Immunol. 1983 Jan;20(1):39-46. doi: 10.1016/0161-5890(83)90103-7.
Hybrid immunoglobulin molecules were constructed from the isolated heavy and light chains of monoclonal anti-arsonate antibodies which differed in their expression of a cross-reactive idiotype. The reconstructed molecules were tested for public and private idiotypic determinants associated with the A-strain response to the hapten p-azophenylarsonate and it was found that both an appropriate heavy chain and an appropriate light chain were required for expression of idiotypic determinants. While appropriate heavy chains could be derived only from idiotype-positive antibodies, appropriate light chains could be derived not only from idiotype positive antibodies, but also from certain idiotype-negative molecules. Similarly, recombinant (hybrid) molecules constructed from two idiotype-positive immunoglobulins differing quantitatively in the degree of idiotypic character, expressed the character at a level which correlated with that of the heavy chain donor. Thus, while the serological expression of idiotypic determinants occurs only in the context of the appropriate VHVL combination, these data demonstrate that the determinants comprising the major cross-reactive idiotype of the anti-arsonate response of A/J mice have their bases in heavy chain structures.
杂合免疫球蛋白分子由单克隆抗砷酸盐抗体的分离重链和轻链构建而成，这些抗体在交叉反应独特型的表达上存在差异。对重建分子进行了与A品系对半抗原对氨基苯砷酸盐反应相关的公共和私有独特型决定簇的检测，发现独特型决定簇的表达需要合适的重链和合适的轻链。虽然合适的重链只能来自独特型阳性抗体，但合适的轻链不仅可以来自独特型阳性抗体，还可以来自某些独特型阴性分子。同样，由两种在独特型特征程度上存在数量差异的独特型阳性免疫球蛋白构建的重组（杂合）分子，表达的特征水平与重链供体的水平相关。因此，虽然独特型决定簇的血清学表达仅在合适的VHVL组合的背景下发生，但这些数据表明，构成A/J小鼠抗砷酸盐反应主要交叉反应独特型的决定簇基于重链结构。
