小鼠集落刺激因子淋巴细胞信使核糖核酸的翻译
Translation of lymphocyte mRNA for mouse colony stimulating factor.
作者信息
Bleackley R C, Horak H, McElhaney J, Havele C, Shaw A R, Turner A R, Paetkau V H
出版信息
Nucleic Acids Res. 1983 May 25;11(10):3027-35. doi: 10.1093/nar/11.10.3027.
Poly A+ RNA has been purified from phorbol myristate acetate stimulated mouse EL4 cells. Translation, by microinjection into frog oocytes, gave biologically active interleukin 2 (IL2) and colony stimulating factor for granulocytes and macrophages (GM-CSF). These two mRNAs were separated by centrifugation through linear sucrose gradients. The sedimentation coefficients for IL2 and GM-CSF mRNAs were found to be 11.5S and 8S respectively. The clonal and cellular morphologies induced by the oocyte material corresponded to low concentrations of authentic GM-CSF.
已从佛波酯肉豆蔻酸酯乙酸盐刺激的小鼠EL4细胞中纯化出聚腺苷酸加尾RNA（Poly A+ RNA）。通过显微注射到蛙卵母细胞中进行翻译，产生了具有生物活性的白细胞介素2（IL2）和粒细胞巨噬细胞集落刺激因子（GM-CSF）。通过线性蔗糖梯度离心分离出这两种信使核糖核酸（mRNA）。发现IL2和GM-CSF信使核糖核酸的沉降系数分别为11.5S和8S。卵母细胞物质诱导的克隆和细胞形态与低浓度的天然GM-CSF相对应。
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