中间丝及其与膜的相互作用。桥粒 - 细胞角蛋白丝复合物与上皮分化。

Intermediate filaments and their interaction with membranes. The desmosome-cytokeratin filament complex and epithelial differentiation.

作者信息

Moll R, Franke W W

出版信息

Pathol Res Pract. 1982;175(2-3):146-61. doi: 10.1016/S0344-0338(82)80105-2.

PMID:6190143

Abstract

Intermediate-sized filaments represent a class of morphologically similar but biochemically and immunologically distinguishable cytoplasmic protein polymer structures. Five major filament types have been identified (cytokeratin, vimentin, desmin, neurofilament protein, glia filament protein) and antibodies to these proteins have been used for distinguishing different cell types and tumors derived therefrom. Epithelial and carcinoma cells are characterized by the presence of cytokeratin filaments and desmosomal elements identified by antibodies to certain high molecular weight proteins of desmosomal plaques. However, the specific pattern of cytokeratin polypeptides is different in different epithelia. The potential value of cell type identification by immunological reactions with antibodies to cytoskeletal proteins in tumor diagnosis is discussed.

摘要

中等大小的丝状物代表一类形态相似但在生化和免疫方面可区分的细胞质蛋白聚合物结构。已鉴定出五种主要的丝状物类型（细胞角蛋白、波形蛋白、结蛋白、神经丝蛋白、胶质丝蛋白），并且针对这些蛋白的抗体已被用于区分不同的细胞类型以及由此衍生的肿瘤。上皮细胞和癌细胞的特征是存在细胞角蛋白丝状物以及通过针对桥粒斑某些高分子量蛋白的抗体鉴定出的桥粒成分。然而，不同上皮中细胞角蛋白多肽的具体模式有所不同。本文讨论了在肿瘤诊断中通过与细胞骨架蛋白抗体进行免疫反应来鉴定细胞类型的潜在价值。