8-氮杂鸟嘌呤抗性非洲绿猴肾细胞突变体（Vero 153）：分离与鉴定

8-Azaguanine resistant African green monkey kidney cell mutants (Vero 153): isolation and characterization.

作者信息

Lee C L, Lee S H, Rozee K R, Chen S H

出版信息

In Vitro. 1983 May;19(5):416-20. doi: 10.1007/BF02619558.

DOI:10.1007/BF02619558

PMID:6190732

Abstract

A clone of Vero cells resistant to up to 20 micrograms/ml 8-azaguanine was isolated. This clone (designated Vero 153) has a doubling rate of approximately 24 h and a maximum cell density of 10,000/mm2. Deficiency of the enzyme hypoxanthine guanine phosphoribosyl transferase (HGPRT) in Vero 153 was demonstrated by methods of radiochromatography. Vero 153 is susceptible to hypoxanthine-thymidine-aminopterin (HAT) medium and its resistance to 8-azaguanine seems to be nonreversible. Like parental cells, Vero 153 was also incapable of interferon production when challenged with Newcastle disease virus (NDV) or poly(inosinic acid) . poly(cytidylic acid) (poly I:C). Similar chromosome complements (majority range 56 to 57) and band patterns were observed in cells harvested at Passages 10, 20, and 50. The potential use of Vero 153 for somatic cell hybridization for purposes of gene mapping, virus rescue, and the control of interferon production is discussed.

摘要

分离出了对高达20微克/毫升8-氮杂鸟嘌呤具有抗性的Vero细胞克隆。该克隆（命名为Vero 153）的倍增时间约为24小时，最大细胞密度为10,000个/平方毫米。通过放射色谱法证实了Vero 153中次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）的缺陷。Vero 153对次黄嘌呤-胸腺嘧啶核苷-氨基蝶呤（HAT）培养基敏感，其对8-氮杂鸟嘌呤的抗性似乎是不可逆的。与亲代细胞一样，当用新城疫病毒（NDV）或聚肌苷酸-聚胞苷酸（poly I:C）刺激时，Vero 153也不能产生干扰素。在第10、20和50代收获的细胞中观察到了相似的染色体组（多数范围为56至57）和带型。讨论了Vero 153在用于基因定位、病毒拯救和干扰素产生控制的体细胞杂交中的潜在用途。