[Establishment of mutant strain from human lung cancer cell (A549) with characteristics of resistance to 8-AG and HGPRT deficiency].

A human lung cancer cell line (A549) mutant with hypoxanthine guanine phosphoribosyltransferase (HGPRT) deficiency and resistance to 8-AG was established by treatment of the A549 cells with 3 micrograms.ml-1 N-methyl-N'-nitro-nitrosoguanidine (MNNG), and prescreened in 0.25% agarose DMEM semisolid medium containing 20 micrograms.ml-1 8-AG. The mutant cells are resistant to cytotoxic effect of 8-AG and sensitive to hypoxanthine-aminopterin thymidine (HAT) selective medium. The mutant cells can be used as a candidate parental cells to fuse with the somatic cells.