Xiao Y, Hu C, Shi K, Luo M
Department of Microbiology, Hunan Medical University, Changsha.
Hunan Yi Ke Da Xue Xue Bao. 1997;22(2):113-5.
A human lung cancer cell line (A549) mutant with hypoxanthine guanine phosphoribosyltransferase (HGPRT) deficiency and resistance to 8-AG was established by treatment of the A549 cells with 3 micrograms.ml-1 N-methyl-N'-nitro-nitrosoguanidine (MNNG), and prescreened in 0.25% agarose DMEM semisolid medium containing 20 micrograms.ml-1 8-AG. The mutant cells are resistant to cytotoxic effect of 8-AG and sensitive to hypoxanthine-aminopterin thymidine (HAT) selective medium. The mutant cells can be used as a candidate parental cells to fuse with the somatic cells.
通过用3微克/毫升的N-甲基-N'-硝基-N-亚硝基胍（MNNG）处理A549细胞，建立了一种次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）缺陷且对8-氮杂鸟嘌呤（8-AG）耐药的人肺癌细胞系（A549），并在含有20微克/毫升8-AG的0.25%琼脂糖DMEM半固体培养基中进行预筛选。突变细胞对8-AG的细胞毒性作用具有抗性，对次黄嘌呤-氨基蝶呤-胸腺嘧啶核苷（HAT）选择培养基敏感。该突变细胞可作为与体细胞融合的候选亲本细胞。
