与肌动蛋白分子取向相关的肌动蛋白丝的一致图像。
A consistent picture of the actin filament related to the orientation of the actin molecule.
作者信息
Fowler W E, Aebi U
出版信息
J Cell Biol. 1983 Jul;97(1):264-9. doi: 10.1083/jcb.97.1.264.
Abstract
We show that freeze-dried actin filaments which have been rotary shadowed with a light coat of platinum appear very similar in morphology and width to negatively-stained filaments. The addition of a thicker coat of platinum to such preparations gives the actin filaments a different morphology and width, which are similar to those of the rotary-shadowed, quick-frozen filaments described by Heuser and Kirschner (J. Cell Biol. 1980, 86:212-234). The consistent view of the actin filament presented here, particularly its 7-8-nm width, can be interpreted in terms of the overall orientation of the actin subunit in the actin filament.
摘要
我们发现，用一层薄铂进行旋转投影的冻干肌动蛋白丝在形态和宽度上与负染色的丝非常相似。在此类制剂上添加一层更厚的铂会使肌动蛋白丝呈现出不同的形态和宽度，这与霍伊泽尔和基施纳（《细胞生物学杂志》，1980年，86卷：212 - 234页）所描述的旋转投影、快速冷冻的丝相似。此处呈现的关于肌动蛋白丝的一致观点，尤其是其7 - 8纳米的宽度，可以根据肌动蛋白亚基在肌动蛋白丝中的整体取向来解释。
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本文引用的文献
1
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2
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J Cell Biol. 1981 Nov;91(2 Pt 1):340-51. doi: 10.1083/jcb.91.2.340.
3
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Nature. 1982 Jul 8;298(5870):131-5. doi: 10.1038/298131a0.
4
Polymorphism of actin paracrystals induced by polylysine.聚赖氨酸诱导的肌动蛋白副晶体的多态性
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5
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