一种从副粘病毒储备液中去除支原体污染的简单方法。

A simple method for the removal of mycoplasma contamination from paramyxovirus stocks.

作者信息

Simpson E J, Cosby S L, Rima B K, Martin S J

出版信息

J Virol Methods. 1983 Mar;6(3):127-34. doi: 10.1016/0166-0934(83)90024-1.

PMID:6190834

Abstract

A simple and quick method of detecting mycoplasmas in virus stocks using the fluorochrome Hoechst 33258 is described. Different methods of removal of mycoplasmas from stocks are discussed. The simple but effective method using gentamicin (0.2 mg/ml) or chloramphenicol (5 micrograms/ml) is demonstrated and chosen as the most efficient as judged using both the Hoechst stain and the direct assessment of mycoplasma RNA species labelled with [5-3H]uridine.

摘要

本文描述了一种使用荧光染料Hoechst 33258检测病毒储备液中支原体的简单快速方法。讨论了从储备液中去除支原体的不同方法。展示了使用庆大霉素（0.2mg/ml）或氯霉素（5μg/ml）的简单但有效的方法，并根据Hoechst染色和用[5-³H]尿苷标记的支原体RNA种类的直接评估判断，该方法被选为最有效的方法。