具有不同小脑细胞构筑的脊椎动物浦肯野细胞群的福尔根DNA模式。

Feulgen DNA pattern of Purkinje cells population in vertebrates with different cerebellar cytoarchitectonics.

作者信息

Bernocchi G

出版信息

J Hirnforsch. 1983;24(1):35-42.

PMID:6190858

Abstract

Quantitative evaluations of Feulgen DNA contents were carried out at different hydrolysis times on Purkinje cell population and granular cells (2c controls) from catfish, frog and quail cerebella. At some hydrolysis times, the blood erythrocytes (2c controls) were examined too. The Feulgen DNA heterogeneity (2c, H2c, 4c) previously found in the rat, was observed in catfish and quail and it was clearly dependent on the different hydrolysis times. On the contrary in the frog, H2c and 4c values only were found. The analysis of the Feulgen hydrolysis kinetics showed a greater homogeneity of chromatin in frog Purkinje cells in comparison to the other species studied. From a comparative standpoint the patterns of Feulgen DNA of Purkinje cells were discussed in relation to the cerebellar size and structural complexity and its "functional importance".

摘要

在不同水解时间对鲶鱼、青蛙和鹌鹑小脑的浦肯野细胞群和颗粒细胞（2c对照）进行了福尔根DNA含量的定量评估。在某些水解时间，还对血液红细胞（2c对照）进行了检查。先前在大鼠中发现的福尔根DNA异质性（2c、H2c、4c）在鲶鱼和鹌鹑中也观察到，并且明显依赖于不同的水解时间。相反，在青蛙中，仅发现了H2c和4c值。福尔根水解动力学分析表明，与其他研究物种相比，青蛙浦肯野细胞中的染色质具有更高的同质性。从比较的角度讨论了浦肯野细胞福尔根DNA模式与小脑大小、结构复杂性及其“功能重要性”的关系。