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用不同细胞化学方法测定某些小脑浦肯野细胞核中的DNA剩余量。

Determination of DNA surplus in the nuclei of some cerebellar Purkinje cells with different cytochemical methods.

作者信息

Brodsky W J, Kudryavtsev B N, Marshak T L

出版信息

Basic Appl Histochem. 1980;24(4):401-8.

PMID:6160846
Abstract

Cytofluorometry and cytophotometry (Feulgen staining) as well as ultraviolet cytophotometry revealed similar histograms of DNA content in squashed nuclei of rat cerebellar Purkinje cells. More than 90% diploid (2c) cells and 1% tetraploid (4c) cells were obtained. DNA content in other Purkinje cells ranged between the 2c and 4c level.

摘要

细胞荧光测定法、细胞光度测定法(福尔根染色法)以及紫外细胞光度测定法显示,大鼠小脑浦肯野细胞压片核中的DNA含量直方图相似。获得了超过90%的二倍体(2c)细胞和1%的四倍体(4c)细胞。其他浦肯野细胞的DNA含量介于2c和4c水平之间。

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