Meuer S C, Hodgdon J C, Cooper D A, Hussey R E, Fitzgerald K A, Schlossman S F, Reinherz E L
J Immunol. 1983 Jul;131(1):186-90.
Human cytotoxic T cell clones were generated against autologous EBV-transformed B lymphocytes. Whereas the majority of the clones expressed the T8 surface glycoproteins and showed a specificity for class I MHC gene products on the target cell, a minority expressed the T4 surface glycoprotein and demonstrated a class II specificity. Monoclonal antibodies to T4 and T8 inhibited cytotoxic effector function of reactive clones in a fashion analogous to their effect on alloreactive CTL clones. Each autoreactive T cell clone was cytotoxic for EBV-transformed B lymphocytes but not pokeweed mitogen-activated or resting autologous lymphocytes, suggesting a dual specificity for an MHC gene product as well as an antigen induced and/or encoded by virus. Taken together, the present findings provide further support for the notion that T4 and T8 serve as associative recognition elements on T lymphocytes for MHC gene products.
针对自体EB病毒转化的B淋巴细胞产生了人细胞毒性T细胞克隆。大多数克隆表达T8表面糖蛋白，并对靶细胞上的I类MHC基因产物具有特异性，而少数克隆表达T4表面糖蛋白，并表现出II类特异性。针对T4和T8的单克隆抗体以类似于它们对同种异体反应性CTL克隆的作用方式抑制反应性克隆的细胞毒性效应功能。每个自身反应性T细胞克隆对EB病毒转化的B淋巴细胞具有细胞毒性，但对商陆丝裂原激活的或静止的自体淋巴细胞没有细胞毒性，这表明对MHC基因产物以及病毒诱导和/或编码的抗原具有双重特异性。综上所述，目前的研究结果为T4和T8作为T淋巴细胞上MHC基因产物的关联识别元件这一观点提供了进一步的支持。
