Esponda P, Souto-Padrón T, De Souza W
J Protozool. 1983 Feb;30(1):105-10. doi: 10.1111/j.1550-7408.1983.tb01041.x.
Ultrastructural cytochemical techniques were used to analyze the nucleus and the kinetoplast of epimastigotes of Trypanosoma cruzi. With the use of ethanolic phosphotungstic acid, which detects basic proteins, reaction product was seen in the chromatin and at the periphery of the kinetoplast. Thallium alcoholate, which interacts with DNA, stained strongly the whole kinetoplast and the chromatin. With the use of a silver impregnation method that detects acidic nucleolar proteins, silver granules were seen preferentially located in the central region of the nucleolus. With the EDTA method, which reveals the presence of ribonucleoproteins, staining was observed in the nuclear pores. Also 6-8 nm fibrils, 25 nm and 40 nm granules, which correspond to the perichromatin fibers, interchromatin granules and the perichromatin granules, respectively, were identified in the nucleus. The EDTA method also revealed the presence of 40 nm granules in the kinetoplast. These granules were seen mainly at the two extremities of the kinetoplast. Freeze-fracture images indicate that the nuclear membrane contains ca. 9 pores/microns2 of nuclear surface area. The mean diameter of the pores was 80 nm. All these results suggest that epimastigotes of T. cruzi have a very active nucleus and a high rate of nucleocytoplasmic interchange.
采用超微结构细胞化学技术分析克氏锥虫上鞭毛体的细胞核和动基体。使用能检测碱性蛋白的乙醇磷钨酸，在染色质和动基体周边可见反应产物。与DNA相互作用的乙醇铊能强烈染色整个动基体和染色质。使用检测酸性核仁蛋白的银浸染法，可见银颗粒优先位于核仁的中央区域。采用能显示核糖核蛋白存在的EDTA法，在核孔处观察到染色。此外，在细胞核中还鉴定出了分别对应于染色质周边纤维、染色质间颗粒和染色质周边颗粒的6 - 8纳米纤维、25纳米和40纳米颗粒。EDTA法还显示动基体中存在40纳米颗粒。这些颗粒主要见于动基体的两端。冷冻蚀刻图像表明，核膜每平方微米核表面积约有9个孔。孔的平均直径为80纳米。所有这些结果表明，克氏锥虫上鞭毛体具有非常活跃的细胞核和很高的核质交换率。
