密码子诱导的转运RNA结合。转运RNA的一种与接头功能相关的特性？
Codon-induced transfer RNA association. A property of transfer RNA involved in its adaptor function?
作者信息
Labuda D, Pörschke D
出版信息
J Mol Biol. 1983 Jun 15;167(1):205-9. doi: 10.1016/s0022-2836(83)80042-4.
It is shown by equilibrium sedimentation that the binding of cognate codons to tRNAPhe (yeast), tRNAPhe (Escherichia coli), tRNALys, tRNAfMet and of the wobble codon UUU to tRNAPhe (yeast) induces dimerization of codon transfer RNA complexes. Analysis of the sedimentation profiles with a quantitative evaluation of the coupling between sedimentation and association equilibrium provides dimerization constants in the range from 1 X 10(4) to 6 X 10(4) M-1. These results on various tRNAs from different organisms suggest that the codon-induced tRNA association is a general phenomenon. Probably the codon-induced tRNA association facilitates the aminoacyl transfer reaction.
平衡沉降实验表明，同源密码子与酵母苯丙氨酸转运RNA（tRNAPhe）、大肠杆菌苯丙氨酸转运RNA（tRNAPhe）、赖氨酸转运RNA（tRNALys）、甲硫氨酸起始转运RNA（tRNAfMet）的结合，以及摆动密码子UUU与酵母苯丙氨酸转运RNA（tRNAPhe）的结合，都会诱导密码子-转运RNA复合物发生二聚化。通过对沉降曲线进行分析，并对沉降与缔合平衡之间的耦合进行定量评估，得出二聚化常数范围为1×10⁴至6×10⁴ M⁻¹。这些针对来自不同生物体的各种转运RNA的结果表明，密码子诱导的转运RNA缔合是一种普遍现象。密码子诱导的转运RNA缔合可能促进了氨酰基转移反应。
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