Singh B R, Thakur R N, Yadav A N
Folia Haematol Int Mag Klin Morphol Blutforsch. 1976;103(2):216-25.
Some of the blood parameters recorded in an air-breathing eel, Amphipnous cuchia under normal respiratory condition during non-breeding period (September-April) are haemoglobin (Hb) concentration 19.26%, haematocrit value 56.16%, RBC number 1.71 million/mm3, RBC size 18.86 X 9.70 mum, mean corpuscular haemoglobin (MCH) 113.4 ng, mean corpuscular haemoglobin concentration (MCHC) 34.2%, blood sugar 77 mg% and ascorbic acid 0.435 mg%. The higher concentration of haemoglobin (19.26%) appears to be related to its obligatory air breathing habit and habitat in a water of low oxygen content. Though a definite trend of increase in the haemoglobin and haematocrit concentration with an increase in the body weight of the fish was lacking, variations were clearly marked related to intrinsic activity of the fish connected with different respiratory conditions. Asphyxiation in a submerged but continuous flow of water (liter/h) for 5 1/2 h resulted in an increase in the above-mentioned parameters to an appreciable extent. These increases were 0.23 million/mm3 in the number of erythrocytes, 6.16% in haemoglobin concentration, 10% in haematocrit value, 20% in blood sugar and 35% in ascorbic acid content. The mean corpuscular haemoglobin showed a decline of 6.2%. Exclusive aerial breathing for 5 1/2 h also caused 7.4% increase in haemoglobin concentration, 9.4% in haematocrit value, 0.14 million/mm3 in RBC number, 20% in blood sugar level, 9% in ascorbic acid content but almost no change in mean corpuscular haemoglobin. The average surface area for diffusion of gases appeared to have reduced by 6.8 mum2 per RBC.
在非繁殖期（9月至4月）正常呼吸条件下，对用空气呼吸的印度双孔鱼（Amphipnous cuchia）记录的一些血液参数如下：血红蛋白（Hb）浓度为19.26%，血细胞比容值为56.16%，红细胞数量为171万/mm³，红细胞大小为18.86×9.70微米，平均红细胞血红蛋白（MCH）为113.4纳克，平均红细胞血红蛋白浓度（MCHC）为34.2%，血糖为77毫克%，抗坏血酸为0.435毫克%。较高的血红蛋白浓度（19.26%）似乎与其强制性空气呼吸习性以及在低氧含量水中的栖息地有关。尽管随着鱼体重的增加，血红蛋白和血细胞比容浓度没有明显的增加趋势，但与鱼在不同呼吸条件下的内在活动相关的变化却很明显。在淹没但持续流水（升/小时）的情况下窒息5.5小时，导致上述参数有相当程度的增加。这些增加包括红细胞数量增加0.23万/mm³、血红蛋白浓度增加6.16%、血细胞比容值增加10%、血糖增加20%以及抗坏血酸含量增加35%。平均红细胞血红蛋白下降了6.2%。仅进行5.5小时的空气呼吸也会使血红蛋白浓度增加7.4%、血细胞比容值增加9.4%、红细胞数量增加0.14万/mm³、血糖水平增加20%、抗坏血酸含量增加9%，但平均红细胞血红蛋白几乎没有变化。每个红细胞的气体扩散平均表面积似乎减少了6.8平方微米。
