Staquet M J, Faure M R, Reano A, Viac J, Thivolet J
Arch Dermatol Res. 1983;275(2):124-9. doi: 10.1007/BF00412888.
The variations in the expression of epidermal keratins occurring during retinoid therapy were studied in patients with psoriasis before and during oral administration of etretinate (aromatic retinoid Ro 10-9359) and compared with normal epidermis. Sodium dodecyl sulfate polyacrylamide gel electrophoresis densitometric readings were performed on keratins extracted from epidermal cells obtained through trypsinization of skin specimens. Epidermal cells were also tested by immunofluorescence (IF) for the presence of BMZ antigens as markers of basal cells and for the presence of TK and KP (67 K, 63 K, and 55 K) with sera with antibodies against BMZ antigens and specific antisera for TK and KP. In psoriasis-involved epidermis, SDS-PAGE anaLysis showed lower amounts of 67 K and increased amounts of 63 K and 55 K, as compared with normal epidermis. Low proportions of cells expressing by IF the 67 K and the 63 K were also noted, with a defective expression of these two KP by suprabasal keratinocytes in psoriasis-involved epidermis. During etretinate administration, a return toward the normal electrophoretic pattern and a correction of the defective cellular expression of these two KP were obtained parallel with clinical improvement. These findings indicate the presence in involved psoriatic epidermis of a population of suprabasal keratinocytes that do not express the high-molecular-weight KP and show a normalization of the relative proportions of the KP with etretinate.
在口服依曲替酯(芳香维甲酸Ro 10-9359)之前及期间,对银屑病患者维甲酸治疗过程中发生的表皮角蛋白表达变化进行了研究,并与正常表皮进行比较。对通过胰蛋白酶消化皮肤标本获得的表皮细胞中提取的角蛋白进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳光密度读数分析。还通过免疫荧光(IF)检测表皮细胞中作为基底细胞标志物的BMZ抗原的存在情况,以及使用抗BMZ抗原血清和针对TK及KP(67K、63K和55K)的特异性抗血清检测TK和KP的存在情况。在银屑病受累表皮中,与正常表皮相比,SDS-PAGE分析显示67K含量较低,63K和55K含量增加。IF检测还发现表达67K和63K的细胞比例较低,在银屑病受累表皮中,这两种角蛋白在基底上层角质形成细胞中的表达存在缺陷。在服用依曲替酯期间,随着临床症状改善,电泳图谱恢复正常,这两种角蛋白的细胞表达缺陷也得到纠正。这些发现表明,在银屑病受累表皮中存在一群基底上层角质形成细胞,它们不表达高分子量角蛋白,且依曲替酯可使角蛋白的相对比例恢复正常。