Haftek M, Faure M, Schmitt D, Thivolet J
J Invest Dermatol. 1983 Jul;81(1):10-4. doi: 10.1111/1523-1747.ep12537454.
Using a monoclonal antibody against human HLA-DR antigens and OKT6, we investigated by indirect immunofluorescence the distribution of Langerhans cells in normal human skin and involved and uninvolved skin from patients with psoriasis before, during, and after systemic aromatic retinoid administration. In parallel, enumeration of HLA-DR and of OKT6+ cells was also performed. In involved psoriatic epidermis the distribution of positive cells was disturbed; OKT6+ cells were reduced in number, as were HLA-DR+ cells which were seen in clusters. In control skin sections, a regular pattern of fluorescent dendritic epidermal cells was noted. In normal-appearing human skin, in nonlesional psoriatic skin, but not in diseased psoriatic skin, the number of OKT6+ cells per epidermal section surface unit was higher than that of HLA-DR expressing cells. Changes in the number and distribution of OKT6 and HLA-DR+ cells in psoriatic involved epidermis were corrected by oral retinoid treatment.
我们使用抗人HLA - DR抗原的单克隆抗体和OKT6，通过间接免疫荧光法研究了正常人类皮肤以及银屑病患者在全身应用芳香维甲酸之前、期间和之后受累及未受累皮肤中朗格汉斯细胞的分布情况。同时，还对HLA - DR和OKT6 +细胞进行了计数。在银屑病受累表皮中，阳性细胞的分布受到干扰；OKT6 +细胞数量减少，HLA - DR +细胞也减少且呈簇状分布。在对照皮肤切片中，观察到荧光树突状表皮细胞呈规则分布。在外观正常的人类皮肤、银屑病非皮损皮肤中，但不在患病的银屑病皮肤中，每个表皮切片表面单位的OKT6 +细胞数量高于表达HLA - DR的细胞数量。口服维甲酸治疗可纠正银屑病受累表皮中OKT6和HLA - DR +细胞数量及分布的变化。
