Vilamitjana J, Baltz T, Baltz D, Barreau C
FEBS Lett. 1983 Jul 25;158(2):343-8. doi: 10.1016/0014-5793(83)80610-3.
Monoclonal antibodies directed against RNA polymerase B of the fungus Podospora comata were selected on the basis of different subunits recognition and inhibitory effect on enzyme activity. A library of 10 antibodies biased toward B180, B145, B39, B23,5 and B11 subunits was constructed. Most of these antibodies also recognize yeast, wheat germ and calf thymus RNA polymerase B. Subunits bearing antigenic determinants are not always homologous in Podospora and yeast enzyme. As some of these antibodies strongly inhibit enzyme activity they constitute potent probes for functional studies of corresponding subunits.
基于对不同亚基的识别以及对酶活性的抑制作用，筛选出了针对真菌匐柄霉RNA聚合酶B的单克隆抗体。构建了一个偏向于B180、B145、B39、B23.5和B11亚基的10种抗体文库。这些抗体中的大多数也能识别酵母、小麦胚芽和小牛胸腺RNA聚合酶B。携带抗原决定簇的亚基在匐柄霉和酵母酶中并不总是同源的。由于其中一些抗体强烈抑制酶活性，它们构成了用于相应亚基功能研究的有效探针。
