Yohe H C, Jacobson R I, Yu R K
J Neurosci Res. 1983;9(4):401-12. doi: 10.1002/jnr.490090406.
The ability of acidic phospho- and sphingolipids to interact with basic proteins was studied by double diffusion analysis. The phospholipids, tri- and diphosphoinositide, and the sphingolipid, sulfatide, interacted with myelin basic protein as evidenced by precipitin line formation. Of the sialoglycosphingolipids (gangliosides) tested, only the myelin-specific monosialoganglioside, GM4, formed a precipitin line with myelin basic protein. In addition, myelin basic protein retarded the activity of Clostridium perfringens neuraminidase against GM4 and the disialoganglioside, GD1b. Examination of purified rat brain myelin suggested the presence of a neuraminidase activity intrinsic to myelin. This finding, in concert with ganglioside-myelin basic protein complexes which selectively protect against neuraminidase, may provide a physiological explanation for the simplified ganglioside pattern found in myelin.
通过双向扩散分析研究了酸性磷酸脂和鞘脂与碱性蛋白质相互作用的能力。磷脂、三磷酸肌醇和二磷酸肌醇以及鞘脂硫苷脂与髓鞘碱性蛋白相互作用，沉淀线的形成证明了这一点。在所测试的唾液酸糖鞘脂（神经节苷脂）中，只有髓鞘特异性单唾液酸神经节苷脂GM4与髓鞘碱性蛋白形成沉淀线。此外，髓鞘碱性蛋白抑制了产气荚膜梭菌神经氨酸酶对GM4和二唾液酸神经节苷脂GD1b的活性。对纯化的大鼠脑髓鞘的检测表明髓鞘存在内在的神经氨酸酶活性。这一发现与能选择性抵抗神经氨酸酶的神经节苷脂 - 髓鞘碱性蛋白复合物相结合，可能为髓鞘中发现的简化神经节苷脂模式提供生理学解释。
