地衣芽孢杆菌膜结合核糖体合成α-淀粉酶和α-葡萄糖苷酶。

Synthesis of alpha-amylase and alpha-glucosidase by membrane bound ribosomes from Bacillus licheniformis.

作者信息

Thirunavukkarasu M, Priest F G

出版信息

Biochem Biophys Res Commun. 1983 Jul 29;114(2):677-83. doi: 10.1016/0006-291x(83)90834-3.

PMID:6192821

Abstract

Alpha-glucosidase was membrane bound during exponential growth of Bacillus licheniformis but was released into the medium during stationary phase. It could be partially removed from exponential phase cells by washing with NaCl (0.5 M). Alpha-Amylase was exclusively extracellular and could not be detected in cells. Polysomes were prepared from exponential phase cells and separated into membrane bound and soluble fractions. In vitro chain completion and immunoprecipitation showed that alpha-glucosidase and alpha-amylase were synthesized by membrane bound and not by soluble ribosomes.

摘要

在地衣芽孢杆菌指数生长期，α-葡萄糖苷酶与细胞膜结合，但在稳定期释放到培养基中。用0.5M NaCl洗涤指数生长期的细胞，可部分去除α-葡萄糖苷酶。α-淀粉酶完全位于细胞外，在细胞中无法检测到。从指数生长期的细胞中制备多核糖体，并分离成膜结合部分和可溶性部分。体外链延伸和免疫沉淀表明，α-葡萄糖苷酶和α-淀粉酶是由膜结合核糖体而非可溶性核糖体合成的。