Rozengurt E, Stroobant P, Waterfield M D, Deuel T F, Keehan M
Cell. 1983 Aug;34(1):265-72. doi: 10.1016/0092-8674(83)90157-5.
Partially purified porcine PDGF or purified human PDGF in the presence of phosphodiesterase inhibitors caused marked accumulation of cAMP in Swiss 3T3 cells. The responses were time- and dose-dependent; half-maximal effect was obtained at 0.6 nM PDGF. Indomethacin prevented the increase of cAMP levels in a dose-dependent manner; half-maximal effect was obtained at about 10 nM. Addition of PDGF increased (at least 25-fold) the production of E-type prostaglandins; PGE reached a concentration in the medium of 26 ng/ml 1 hr after treatment with human PDGF. This concentration of PGE produced a similar level of cAMP to that found with PDGF, suggesting that the PDGF-induced increase in cAMP is mediated by E-type prostaglandins released in the culture medium. Increased cAMP levels promoted by PDGF acting through stimulation of E-type prostaglandin synthesis may contribute to signal the initiation of cell proliferation in 3T3 cells.
在磷酸二酯酶抑制剂存在的情况下，部分纯化的猪血小板衍生生长因子（PDGF）或纯化的人PDGF可导致瑞士3T3细胞中cAMP显著积累。这些反应具有时间和剂量依赖性；在0.6 nM PDGF时可获得半数最大效应。吲哚美辛以剂量依赖性方式阻止cAMP水平升高；在约10 nM时可获得半数最大效应。添加PDGF可使E型前列腺素的产生增加（至少25倍）；用人PDGF处理1小时后，培养基中PGE的浓度达到26 ng/ml。这种PGE浓度产生的cAMP水平与PDGF作用时相似，表明PDGF诱导的cAMP增加是由培养基中释放的E型前列腺素介导的。通过刺激E型前列腺素合成起作用的PDGF所促进的cAMP水平升高，可能有助于为3T3细胞中细胞增殖的启动发出信号。
