Mitrovic D, Quintero M, Stankovic A, Ryckewaert A
Lab Invest. 1983 Sep;49(3):309-16.
Cell and clonal density, lacunar and clonal diameters, and mean number of cells per single clone were studied in human femoral condylar cartilage of normal and osteoarthrotic joints. The values were related to the age of the subjects, the sampling site within the joints, and the depth from the articular surface. Cell density in every zone of both normal and diseased tissue decreased with increasing distance from the surface and increasing age. Cell density was significantly lower in age-matched osteoarthritic articular cartilage with an intact surface than in that of normal joints. The age-related decrease in the cell density accompanied an increase in the density of empty lacunae. The density of Alcian blue-stained cells, which actively synthesize proteoglycans as demonstrated by histoautoradiography, diminished with advancing age in all zones of articular cartilage. The percentage of these cells consistently decreased in the superficial and increased in the deep layers of the tissue in both normal and osteoarthritic joints. The clonal density was higher in the nonweight-bearing than in weight-bearing areas, and it increased with age, whereas the mean clone diameter and clonal cell number decreased.
对正常关节和骨关节炎关节的人股骨髁软骨中的细胞和克隆密度、陷窝和克隆直径以及每个单克隆的平均细胞数进行了研究。这些数值与受试者的年龄、关节内的取样部位以及距关节表面的深度有关。正常组织和病变组织的每个区域中的细胞密度均随着距表面距离的增加和年龄的增长而降低。与年龄匹配的表面完整的骨关节炎关节软骨中的细胞密度显著低于正常关节。细胞密度随年龄的下降伴随着空陷窝密度的增加。组织放射自显影显示，能活跃合成蛋白聚糖的阿尔新蓝染色细胞的密度在关节软骨的所有区域均随着年龄的增长而降低。在正常关节和骨关节炎关节中，这些细胞的百分比在组织浅层持续下降，而在深层则增加。非负重区域的克隆密度高于负重区域，并且随年龄增加，而平均克隆直径和克隆细胞数则减少。
