Quintero M, Mitrovic D R, Stankovic M A, de Sèze S, Miravet L, Ryckewaert A
Rev Rhum Mal Osteoartic. 1984 Oct;51(9):445-9.
The authors studied the cellular density (number of cells per mm2) of cartilage taken from the femoral condyles of 46 cadavers (73 knees). In each case, the cartilage was taken from the summit of the condyle and, in 46 joints (27 subjects), a sample was also taken from the posterior surface (non weight-bearing zones). The fragments of non-calcified cartilage were sectioned with a cryostat and the sections (10 micrometers) were stained with hematein-eosin and solid red-Alcian blue. This study demonstrates: 1) a decrease in the cellular density of fissured cartilage compared to normal cartilage; this decrease appears to be proportional to the degree of fibrillation; 2) a decrease in the cellular density of apparently normal cartilage from arthrotic joints compared with normal cartilage from healthy joints; 3) with the age of the subjects, an increase in the density of the clones (number of clones per mm2) and the density of clonal cells (number of clonal cells per mm2) together with a decrease in the mean number of chondrocytes per clone. In fissured cartilage, the density of the clones, the density of the clonal cells and the number of chondrocytes per clone are slightly higher on the posterior surface of the condyles than on the summit of the condyles. These results emphasise the importance of the role that might be played by cellular phenomena in the mechanisms of deterioration of cartilage with aging and with arthrosis.
作者研究了取自46具尸体（73个膝关节）股骨髁的软骨细胞密度（每平方毫米细胞数）。在每种情况下，软骨均取自髁的顶部，并且在46个关节（27名受试者）中，还从后表面（非负重区）采集了样本。将未钙化软骨碎片用低温恒温器切片，切片（10微米）用苏木精-伊红和固红-阿尔辛蓝染色。本研究表明：1）与正常软骨相比，裂隙软骨的细胞密度降低；这种降低似乎与纤维化程度成正比；2）与健康关节的正常软骨相比，关节病关节中看似正常的软骨细胞密度降低；3）随着受试者年龄的增长，克隆密度（每平方毫米克隆数）和克隆细胞密度（每平方毫米克隆细胞数）增加，同时每个克隆中软骨细胞的平均数量减少。在裂隙软骨中，髁后表面的克隆密度、克隆细胞密度和每个克隆中的软骨细胞数量略高于髁顶部。这些结果强调了细胞现象在软骨随年龄增长和患关节病而退化的机制中可能发挥的作用的重要性。
