Doctor V M, Sauls V
Thromb Res. 1983 Jun 15;30(6):573-8. doi: 10.1016/0049-3848(83)90265-7.
Larchwood xylan was purified by diaminoethylaminoethyl (DEAE) cellulose chromatography, sulfated by heating with chlorosulfonic acid -pyridine complex and the sulfated polysaccharide was isolated by epichlorohydrin triethanolamine (ECTEOLA) cellulose chromatography as the sodium salt. It's molecular weight was approximately 25000 and the specific rotation was [alpha]D 20-70 degrees. Electrophoretic analysis of the sulfated xylan along with heparin and SP-54 using lithium acetate-agarose technique showed that the charge density of the xylan sulfate was similar to heparin and it moved as a single component in contrast to commercial heparin which resolved into two while SP-54 moved at the same rate as the marker dye. Anticoagulant properties of the sulfated xylan were compared with heparin and SP-54 by studying its effect on the activated partial thromboplastin time (APTT), prothrombin time (PT) and the thrombin time (TT) using pooled normal human plasma. The sulfated xylan was more effective than SP-54 in delaying coagulation by all the three measurements while it was less active than heparin in inhibiting APTT and PT.
落叶松木聚糖通过二氨基乙基氨基乙基（DEAE）纤维素色谱法纯化，用氯磺酸 - 吡啶络合物加热进行硫酸化，然后通过环氧氯丙烷三乙醇胺（ECTEOLA）纤维素色谱法将硫酸化多糖分离为钠盐。其分子量约为25000，比旋光度为[α]D 20 - 70度。使用醋酸锂 - 琼脂糖技术对硫酸化木聚糖以及肝素和SP - 54进行电泳分析表明，硫酸化木聚糖的电荷密度与肝素相似，并且它作为单一成分移动，而商业肝素则分解为两个成分，而SP - 54与标记染料以相同速率移动。通过使用混合正常人血浆研究其对活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）和凝血酶时间（TT）的影响，将硫酸化木聚糖的抗凝特性与肝素和SP - 54进行比较。在所有三项测量中，硫酸化木聚糖在延迟凝血方面比SP - 54更有效，而在抑制APTT和PT方面比肝素活性更低。
