College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
Jiangsu Drug Safety Evaluation Center, Nanjing 211816, China.
Int J Biol Macromol. 2020 Jul 15;155:1460-1467. doi: 10.1016/j.ijbiomac.2019.11.124. Epub 2019 Nov 14.
High-value utilization of hemicellulose is critical to improve the append value of integrated biorefineries. In this research, the alkali-soluble sugarcane bagasse hemicellulose was sulfated using chlorosulfonic acid and N,N-dimethylformamide/LiCl under homogeneous conditions. With the aid of flow technique, a rapid, mild, and efficient method for the synthesis of xylan sulfate with high molecular weight and controllable degree of substitution was achieved. The results showed that the reaction time and the degradation of xylan chain were drastically reduced compared to the "in flask" batch conditions. High molecular weight of the product (M = 148,217) with a reasonable degree of substitution (DS = 1.49) could be obtained even at room temperature in 10 min under the present flow system. Anticoagulant experiments showed good anticoagulant activity of the resultant xylan sulfate, which could significantly prolong the activated partial thromboplastin time and thrombin time. This work not only provides a novel method for the synthesis of xylan sulfate, but also offers new opportunities for the production of other functional polysaccharide derivatives under the flow reaction conditions.
高效利用半纤维素对于提高综合生物精炼厂的附加值至关重要。本研究采用氯磺酸和 N,N-二甲基甲酰胺/氯化锂在均相条件下对碱溶性甘蔗渣半纤维素进行硫酸化。借助流动技术，实现了快速、温和、高效合成具有高分子量和可控取代度的木聚糖硫酸酯的方法。结果表明，与“瓶内”分批条件相比，反应时间和木聚糖链的降解大大缩短。即使在室温下，在当前的流动体系中，仅需 10 分钟即可获得具有合理取代度（DS=1.49）的高分子量产物（M=148217）。抗凝实验表明，所得木聚糖硫酸酯具有良好的抗凝活性，可显著延长活化部分凝血活酶时间和凝血酶时间。这项工作不仅为木聚糖硫酸酯的合成提供了一种新方法，而且为在流动反应条件下生产其他功能性多糖衍生物提供了新的机会。
