van Raaij A J, de Leeuw A L, Janssen A P, Broekhuyse R M
Exp Eye Res. 1983 Aug;37(2):183-90. doi: 10.1016/0014-4835(83)90077-5.
An antiserum has been prepared against the EDTA-extractable proteins (EEP) from calf lens fiber membranes. It was shown to be highly specific for EEP. Using this anti-EEP antiserum in (crossed-line) immunoelectrophoresis and (crossed) immunoelectrofocusing experiments, evidence was obtained that all of the EDTA-extractable proteins are immunologically related. They have at least one of six different antigenic determinants in common, while some of the proteins with pI values above 4.8 probably have a second common determinant. The acidic proteins of EEP comprise specific determinants with a low immunogenicity. Furthermore, it was demonstrated that no crystallin-like determinant was present on the EEP molecules.
