大鼠肝脏、肾脏和脾脏中总DNA和RNA的年龄相关变化以及尿苷和胸苷的掺入情况。

Age-related changes in total DNA and RNA and incorporation of uridine and thymidine in rat liver, kidney and spleen.

作者信息

Messineo L, Denko C W, Petricevic M

出版信息

Int J Biochem. 1983;15(9):1103-7. doi: 10.1016/0020-711x(83)90224-0.

PMID:6194021

Abstract

Total content of DNA and RNA in liver, kidney and spleen were measured in young and aged rats. At the same time the incorporation of [14C]thymidine, a DNA precursor, and [3H]uridine, an RNA precursor, were also determined. Changes in total organ DNA and RNA correlated with sexual maturation as did incorporation of precursors. Young animals have more DNA per organ relative to RNA, with kidney and spleen DNA showing a decrease between maturity and senescence. However, liver RNA increases with age, a change probably due to decreased catabolism of RNA since [3H]urine uptake decreases. Liver polyploid differentiation, and [14C]thymidine and [3H]uridine uptake, are correlated. In kidney, incorporation of [3H]uridine is inversely related to [14C]thymidine incorporation.

摘要

在幼年和老年大鼠中测量了肝脏、肾脏和脾脏中DNA和RNA的总含量。同时还测定了DNA前体[14C]胸腺嘧啶核苷和RNA前体[3H]尿苷的掺入情况。器官DNA和RNA总量的变化与性成熟相关，前体的掺入情况也是如此。相对于RNA，幼年动物每个器官的DNA更多，肾脏和脾脏的DNA在成熟和衰老之间呈现减少趋势。然而，肝脏RNA随年龄增长而增加，这种变化可能是由于RNA分解代谢减少所致，因为[3H]尿苷摄取量减少。肝脏多倍体分化与[14C]胸腺嘧啶核苷和[3H]尿苷摄取相关。在肾脏中，[3H]尿苷的掺入与[14C]胸腺嘧啶核苷的掺入呈负相关。