Lorup C
Cell Tissue Kinet. 1977 Sep;10(5):477-85. doi: 10.1111/j.1365-2184.1977.tb00866.x.
An autoradiographic study was made of the 3H-uridine incorporation into RNA and DNA in nucleus and cytoplasm of parenchymal cells in the regenerating liver of the mouse after a pulse time of 2 hr. After a decreased uptake of precursor into the parenchymal nucleus during the first 6 hr compared with the normal value, incorporation increased and was maximal at 36 hr; normal values were restored at 72 hr. The cytoplasmic labelling, after an initial small decrease, reached a maximum at 12 hr; this changed to normal 48 hr after hepatectomy. RNase-digestion of the liver sections left a small incorporation in both nucleus and cytoplasm: presumably DNA. This incorporation is maximal at 12 hr over the nucleus and at 24 hr over the cytoplasm. After a 2 hr pulse of 3H-thymidine, there was a marked uptake of the precursor into DNA about 24 hr after hepatectomy. This was maximal at 48 hr and reached normal values at 72 hr. A small amount of incorporation of 3H-thymidine into DNA was seen immediately after the operation, and this population of weakly labelled nuclei was still rather large 72 hr later.
对小鼠再生肝实质细胞核与细胞质中3H-尿苷掺入RNA和DNA的情况进行了放射自显影研究，脉冲时间为2小时。与正常值相比，在最初6小时内实质细胞核对前体的摄取减少，之后掺入增加，并在36小时达到最大值；72小时恢复到正常值。细胞质标记在最初略有下降后，于12小时达到最大值；肝切除术后48小时恢复正常。用RNase消化肝切片后，细胞核和细胞质中仍有少量掺入：推测为DNA。这种掺入在细胞核中于12小时达到最大值，在细胞质中于24小时达到最大值。在3H-胸苷脉冲2小时后，肝切除术后约24小时前体明显掺入DNA。这在48小时达到最大值，并在72小时恢复到正常值。手术后立即可见少量3H-胸苷掺入DNA，72小时后这群弱标记的细胞核仍然相当多。
