Smith J H, Eliceiri G L
J Cell Physiol. 1983 Oct;117(1):128-34. doi: 10.1002/jcp.1041170118.
Several observations have been made about the associations of small nuclear RNAs (snRNAs) in human cells. When nuclear RNA was extracted with phenol and chloroform under standard nondenaturing conditions, the proportion of the nuclear snRNA content that cosedimented with high molecular weight RNA was very low. These results do not support the proposal that it is a large percentage of the cellular snRNA content that is involved in relatively stable base-paired interactions with heterogeneous nuclear RNA at any given time. The various small nuclear ribonucleoprotein particles (snRNPs), in which the snRNAs are found in the cell, appear to differ substantially in their sedimentation rates under conditions of physiological ionic strength. Using anti-RNP and anti-Sm antibodies to analyze various subcellular fractions, we found that most, if not all, of the U1 snRNA cellular content is associated with the polypeptide(s) bearing the RNP determinant (in interphase and mitotic cells) and with the polypeptide(s) carrying the Sm determinant (in mitotic cells).
关于人类细胞中小核RNA（snRNA）的关联，已经有了一些观察结果。当在标准非变性条件下用苯酚和氯仿提取核RNA时，与高分子量RNA共沉降的核snRNA含量比例非常低。这些结果不支持这样的提议，即在任何给定时间，有很大比例的细胞snRNA含量参与与不均一核RNA相对稳定的碱基配对相互作用。在细胞中发现snRNA的各种小核核糖核蛋白颗粒（snRNP），在生理离子强度条件下它们的沉降速率似乎有很大差异。使用抗RNP和抗Sm抗体分析各种亚细胞组分，我们发现，U1 snRNA细胞含量中的大部分（如果不是全部的话），在间期和有丝分裂细胞中与带有RNP决定簇的多肽相关联，在有丝分裂细胞中与带有Sm决定簇的多肽相关联。
