Rosen E, Sivertsen A, Firtel R A
Cell. 1983 Nov;35(1):243-51. doi: 10.1016/0092-8674(83)90227-1.
We have isolated and characterized a transposable element from Dictyostelium discoideum denoted Tdd-1. There are approximately 50 complete copies of the element per haploid genome and approximately 100-150 partial elements. Southern blots of DNA from different Dictyostelium discoideum strains show that Tdd-1 is a mobile element. Tdd-1 is 4.9 kb long, with 313 bp inverted repeats. These repeats lie near the termini, but unlike other transposable elements one end of Tdd-1 extends 36 bp past the repeat and the other extends 1 bp. Tdd-1 encodes a series of developmentally regulated transcripts, all with the same polarity, that increase dramatically in abundance after approximately 10 hr of development. We have also identified a heat shock inducible transcript of the opposite polarity.
我们从盘基网柄菌中分离并鉴定了一种转座元件，命名为Tdd-1。单倍体基因组中约有50个该元件的完整拷贝以及约100 - 150个部分元件。来自不同盘基网柄菌菌株的DNA的Southern杂交结果表明Tdd-1是一种可移动元件。Tdd-1长度为4.9 kb，具有313 bp的反向重复序列。这些重复序列位于末端附近，但与其他转座元件不同的是，Tdd-1的一端在重复序列后延伸了36 bp，另一端延伸了1 bp。Tdd-1编码一系列发育调控转录本，所有转录本具有相同的极性，在发育约10小时后丰度急剧增加。我们还鉴定出了一种极性相反的热休克诱导转录本。
