Speer C A, Wong R B, Schenkel R H
J Protozool. 1983 Aug;30(3):548-54. doi: 10.1111/j.1550-7408.1983.tb01420.x.
Monoclonal IgG antibodies against sporozoites of Eimeria tenella were obtained from the ascites fluid of BALB/c mice. Oocysts, sporocysts, and sporozoites were exposed to medium 199, normal ascites fluid, or monoclonal antibodies 1A, 9D, 3D3II, or 2G8f. Specimens were then incubated with ferritin-conjugated goat anti-mouse IgG antibody. Ferritin was uniformly distributed over the surface of sporozoites exposed to 1A, 9D, or 3D3II; ferritin was localized in patches on sporozoites exposed to 2G8f. A uniform layer of ferritin was present on the inner layer of oocyst walls and on the Stieda body, but not on the sporocyst wall. No ferritin was found on specimens exposed to medium 199 or normal ascites fluid. Monoclonal antibodies 1A, 9D, and 3D3II, but not 2G8F, caused complement-mediated lysis of sporozoites. These findings indicate that oocysts, sporocysts, and sporozoites of E. tenella contain common antigens specific for each monoclonal antibody tested.
从BALB/c小鼠腹水中获得了针对柔嫩艾美耳球虫子孢子的单克隆IgG抗体。将卵囊、子孢子囊和子孢子暴露于199培养基、正常腹水或单克隆抗体1A、9D、3D3II或2G8f中。然后将标本与铁蛋白偶联的山羊抗小鼠IgG抗体孵育。铁蛋白均匀分布在暴露于1A、9D或3D3II的子孢子表面；铁蛋白定位于暴露于2G8f的子孢子上的斑块中。卵囊壁内层和斯氏体上有一层均匀的铁蛋白，但子孢子囊壁上没有。在暴露于199培养基或正常腹水的标本上未发现铁蛋白。单克隆抗体1A、9D和3D3II可引起补体介导的子孢子裂解，而2G8F则不能。这些发现表明，柔嫩艾美耳球虫的卵囊、子孢子囊和子孢子含有针对所测试的每种单克隆抗体的共同抗原。
