Callewaert D M, Mahle N H, Dayner S, Earnshaw B, Bowman S, O'Connor M
Scand J Immunol. 1983 Nov;18(5):429-38. doi: 10.1111/j.1365-3083.1983.tb00875.x.
Distribution-free analysis of kinetic data for cellular cytotoxicity reactions enables the precise determination of kinetic parameters for the lysis of target cells by individual lymphocyte preparations. This report presents results obtained when this method was used to quantitate the inhibition of human natural cytotoxicity by unlabelled (cold) target cells. In agreement with previous studies, we found that the natural cytotoxicity of a given target cell can be inhibited by heterologous as well as homologous unlabelled cells; however, the strongest inhibition was usually produced when the unlabelled inhibitor cells were homologous to the labelled target cells. The general pattern of inhibition observed for the target and inhibitor cells tested in these experiments was competitive, and when inhibitor cells were homologous to the labelled cells, the observed increase in Kappm agreed with theoretical predictions based on equations derived previously. These results support earlier reports of limited but not absolute antigenic specificity by subsets of human NK cells. Moreover, while Kappm values for cytotoxicity reactions are not simply related to antigen binding, quantitative analysis of the relative inhibition of cytotoxicity by heterologous versus homologous unlabelled cells provides useful estimates of the relative affinity of the effector cell subset(s) that kill a given target cell for various NK target cells.
对细胞毒性反应动力学数据进行无分布分析，能够精确测定单个淋巴细胞制剂裂解靶细胞的动力学参数。本报告展示了使用该方法定量未标记（冷）靶细胞对人自然细胞毒性的抑制作用时所获得的结果。与先前的研究一致，我们发现给定靶细胞的自然细胞毒性可被异源以及同源未标记细胞抑制；然而，当未标记的抑制细胞与标记的靶细胞同源时，通常会产生最强的抑制作用。在这些实验中测试的靶细胞和抑制细胞所观察到的总体抑制模式是竞争性的，并且当抑制细胞与标记细胞同源时，观察到的Kappm增加与基于先前推导方程的理论预测相符。这些结果支持了早期关于人NK细胞亚群具有有限但非绝对抗原特异性的报道。此外，虽然细胞毒性反应的Kappm值与抗原结合并非简单相关，但对异源与同源未标记细胞对细胞毒性的相对抑制进行定量分析，可为杀伤给定靶细胞的效应细胞亚群对各种NK靶细胞的相对亲和力提供有用的估计。
