Kuhry J G, Fonteneau P, Duportail G, Maechling C, Laustriat G
Cell Biophys. 1983 Jun;5(2):129-40. doi: 10.1007/BF02796139.
Fluorescence intensity measurements and fluorescence microscopy data showed that TMA-DPH (trimethylammonium diphenylhexatriene), a cationic derivative of the fluorescence polarization probe DPH, has a considerably different behavior in L929 cultured cells than does its parent molecule. In contrast to DPH, it incorporates very rapidly in the plasma membranes of the treated cells, and remains specifically localized on the cell surface for at least 25 min. It can therefore be recommended for specific plasma membrane fluidity measurements in whole living cells. No relevant information about the localization of the probes could be obtained by other techniques used in parallel, namely: subcellular fractionation and fluorescence inhibition by trinitrobenzene sulfonate (TNBS).
荧光强度测量和荧光显微镜数据表明,荧光偏振探针DPH的阳离子衍生物TMA-DPH(三甲基铵二苯基己三烯)在L929培养细胞中的行为与其母体分子有很大不同。与DPH相反,它能非常迅速地掺入处理过的细胞的质膜中,并在细胞表面特异性定位至少25分钟。因此,它可推荐用于完整活细胞中特定的质膜流动性测量。通过同时使用的其他技术,即亚细胞分级分离和三硝基苯磺酸(TNBS)荧光抑制,无法获得有关探针定位的相关信息。