Institute of Solid State Physics, Bulgarian Academy of Sciences, Tzarigradsko Chaussee 72, 1784 Sofia, Bulgaria.
Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.
Int J Mol Sci. 2023 Jun 16;24(12):10226. doi: 10.3390/ijms241210226.
Gold nanoparticles (AuNPs) are promising candidates in various biomedical applications such as sensors, imaging, and cancer therapy. Understanding the influence of AuNPs on lipid membranes is important to assure their safety in the biological environment and to improve their scope in nanomedicine. In this regard, the present study aimed to analyze the effects of different concentrations (0.5, 1, and 2 wt.%) of dodecanethiol functionalized hydrophobic AuNPs on the structure and fluidity of zwitterionic 1-stearoyl-2-oleoyl--glycerol-3-phosphocholine (SOPC) lipid bilayer membranes using Fourier-transform infrared (FTIR) spectroscopy and fluorescent spectroscopy. The size of AuNPs was found to be 2.2 ± 1.1 nm using transmission electron microscopy. FTIR results have shown that the AuNPs induced a slight shift in methylene stretching bands, while the band positions of carbonyl and phosphate group stretching were unaffected. Temperature-dependent fluorescent anisotropy measurements showed that the incorporation of AuNPs up to 2 wt.% did not affect the lipid order in membranes. Overall, these results indicate that the hydrophobic AuNPs in the studied concentration did not cause any significant alterations in the structure and membrane fluidity, which suggests the suitability of these particles to form liposome-AuNP hybrids for diverse biomedical applications including drug delivery and therapy.
金纳米粒子（AuNPs）在各种生物医学应用中具有广阔的应用前景，如传感器、成像和癌症治疗等。了解 AuNPs 对脂质膜的影响对于确保其在生物环境中的安全性以及提高其在纳米医学中的应用范围非常重要。在这方面，本研究旨在使用傅里叶变换红外（FTIR）光谱和荧光光谱分析不同浓度（0.5、1 和 2wt%）的十二硫醇功能化疏水性 AuNPs 对两性离子 1-硬脂酰-2-油酰基-甘油-3-磷酸胆碱（SOPC）脂质双层膜结构和流动性的影响。使用透射电子显微镜发现 AuNPs 的尺寸为 2.2±1.1nm。FTIR 结果表明，AuNPs 诱导了亚甲基伸缩带的轻微位移，而羰基和磷酸基团伸缩带的位置不受影响。温度依赖性荧光各向异性测量表明，AuNPs 的掺入量高达 2wt.%，不会影响膜中的脂质有序性。总的来说，这些结果表明，在所研究浓度下的疏水性 AuNPs 不会对结构和膜流动性造成任何显著改变，这表明这些粒子适合形成脂质体-AuNP 杂化物，用于包括药物输送和治疗在内的各种生物医学应用。
